Kamis, 08 Maret 2012

Barfoed Test

Barfoed Test is used to differ upon monosaccharide and disaccharide.
Monosaccharide reducting copper solution in the acid environment, forming red-brown or brown-orange pellet. In the more time heating, reduction disaccharide will show the same color.

Method to make baffoed reagent:
Mix 13,3 gram of copper acetat in the 200 ml of water and add 1,8 ml acetic acid glacial.

Procedure:
1. Take 1 ml solution that will be tested in the test tube
2. Add 2 ml of barfoed reagent
3. Boiled precise 1 minute, wait until pellet appear
4. See the color of pellet.

Positive Test:




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