Kamis, 08 Maret 2012

Seliwanoff Test


Seliwanof Test is a chemical test to detect the presence of ketose sugar.
This test specific to ketose sugar. The basis of teory of the test is formed 4-hidroximetil furfural which reacted with resorsinol (1,3-hidroxibenzene) forming the compound with red color. Glucose can gives cerry red color in the longer time heated. In the high concentration, glucose and another sugar will gives the same color.


Method to make seliwanoff reagent:
dissolve 0,5 gram of resorsinol in the 100 ml of alcohol and add with 5 N HCl.


Procedure:
1. Take 0,5 ml solution which will be tested in the test tube
2. Add 5 ml of HCl
3. Boiled aproximately 10 minutes
4. Look the color of solution.


Reaction:







Positive Test:













Courtesy of en.wikipedia.org

Tidak ada komentar:

Posting Komentar