Millon Test

Milon test is chemical reaction to detect the presence of tyrosine.
It is specific reaction to monophenol derivative as tyrosine. In this test we use mercury ion in the nitric acid. Positive test will  show red color in the solution. The red color formed of salt from tyrosine nitration. Hidroxiphenil from tyrosine will react to Hg from millon reagent to produce red color.

Method to make millon reagent:
1. Take 2,5 gram of mercury sulphat and add with 5 ml HNO3
2. Add solution with 15 ml of aquadest

Procedure:
1. Take 2 ml of solution that will be tested in the test tube
2. Add the solution with 5 drops of millon reagent
3. White floe will seen, heated carefully
4. See the color of solution.