Benedict Test

Benedict Test is a chemical test to detect the presence of monosaccharide and reduction sugar.
Sugar which have aldehide will react with alkali  copper solution to forming cuprokside that colored. Positive reaction happen if the solution color is green, orange-red or red and green, yellow, or red sediment.


Method to make benedict reagent:
1. Dissolve 173 gram Natrium citrat and 200 gram Sodium karbonat (christal) in the 800 ml aquadest, heated to improve reaction rate
2.Filter the solution
3. Dissolve 17,3 gram of cupri sulphat in the 100 ml aquadest, pour to the first solution slowly. Stir continuously, waiting until the solution cold and then add aquadest until the solution reach 1 L.


Procedure of the Test:
1. Take 0,5 ml of solutions which want to be tested in the test tube
2. Add with 5 ml of benedict reagent
3. Boiled aproximatelly in 2 minutes
4. Look at the solution color
5. Waiting until sediment arising out, look at the sediment color.

Result:

Courtesy of www.jce.divched.org