Ninhidrin Test

Ninhidrin test is chemical test to detect the presence of amino acids.
All kinds of amino acid can detected with this test. Amino acid reacted with ninhidrin (triketohidrindenhidrat) to forming aldehide and release Nh3 and CO2. In this reaction also formed blue color (but prolin and hidroxiprolin will show yellow color). The blue color is reaction of 2 molecule of ninhidrin with NH3. It is the signing of positive result for this test.

Procedure:
1. Take 2 ml of solution that will be tested to the test tube
2. Add with 5 drops of ninhidrin solutions
3. Boiled in 10 minutes
4. See the color of solution.