Hopkins-Cole Test

Hopkins-Cole Test is used to detect the presence of tripthopahan.
The reagent contain glioksilic acid. Positive reaction will show purple color (ring) in the solution boundaries. The purple color occur by condensation of 2 core of tripthophan to glioksilic. This test can't succes if there are strong ocsidator like nitrat and clorat. Sulphuric acid that used must very pure, it is means that there aren't other matter which will be an ocsidator.

Procedure:
1. Take 2 ml of solution that will be tested in the test tube
2. Add with 2 ml of Hopkins-Cole reagent
3. Add H2SO4 from the wall of the test tube
4. Wait some seconds
5. See the color of solution.